The archive "MreB in vitro.tar" contains a collection of raw data for the BMC Mol Cell Biol publication of Dersch et al. 2020. 
Data show that B. subtilis MreB paralogs do not easily form ordered filaments in vitro, possibly due to extensive lateral contacts, 
but can co-polymerize. Monomeric MreB, Mbl and MreBH uniformly bind to a membrane, and form irregular and frequently split up filamentous structures, 
facilitated by the addition of divalent ions, and counteracted by monovalent ions, 
suggesting that intracellular potassium levels may be one important factor to counteract extensive filament formation and filament splitting in vivo.

- EM: Electron-Microscope imaging of MreB, YFP-MreB monomers and polymers after addition of divalent ions 
- Membrane-assay protein concentrations: Varying concentrations of YFP-MreB, CFP-Mbl and mCherry-MreBH on an artificial lipid membrane.
- Mix (MreB, Mbl, MreBH): Monomers prior to induction on artificial membrane and co-polymers formed by mixing of the paralogous proteins or by adding them sequentially to existing polymers.
- MreB 10�m + YFP-MreB 0.1�m: Untagged MreB polymers that are made visible through addition of low concentrations of fluorescence-tagged-protein.
- MreB filament rendermovie: 3D-reconstruction of YFP-MreB filaments from STED data.
- MreB in vesicles: MreB structures inside of multilayered veicles.
- MreB monovalent ions: MreB under varying polymerization inhibiting / inducing ion conditions.
- Reaction-Chamber lipid membrane-assay: Planar lipid membrane formation from sonicated vesicles.
- STED: Super-resolution image acquisition of filamentous structures.
- Strep-C-MreB maleimide staining: Maleimide staining of a cysteine mutant MreB to visualize polymerization. 
- Sucrose gradient and blots: Sucrose gradient of monomeric YFP-MreB,CFP-Mbl and mCherry-MreBH and corresponding western blots with specific antibodies against each protein.

Regarding hyperstacks and image formats: 
.nd2 and .lif files are sometimes hyperstack containers with multiple stacks/images.
These files can easily be opened via Fiji, or using the openBioformats extension of imageJ.