Retinal pigment epithelial cell maturation

Subset that includes video data

General information

• Title: Maturation-dependent changes in complement production and complement C3 processing in retinal pigment epithelium cells
• Authors: Juliane Schikora¹, Aaron Dort¹, Antonia Nickel¹, Jasmin Bergert¹, Stella Y. Schayan-Araghi¹, Pratiti Banerjee¹, Hannah N. Wolf¹, Helen May-Simera² and Diana Pauly¹
• Affiliations: ¹Experimental Ophthalmology, University Marburg, Marburg, Germany; ²Institute of Molecular Physiology, Johannes Gutenberg-University, Mainz, Germany

Repository overview

This subset of the repository contains the video data of the study. This includes mp4-files that show stained proteins in an apical to basal direction. It further contains the Fiji macro used for generating mp4-files from tif-files that can be found in the image data subset of this collection.

Experiments were conducted with two cell types, ARPE-19 (adult retinal pigment epithelial cell line) and induced pluripotent stem cell-derived RPE (iPSC-RPE) cells. ARPE-19 and iPSC-RPE cells were each grouped into four condition categories:

• Immature: Cells with an immature, RPE-unspecific phenotype
• Mature: Cells with a mature, RPE-specific phenotype
• Treated: Cells that were treated with 10 ng/mL TGF-Beta1
• Control: Untreated cells

Data collection structure (current subset marked in bold letters)

/ Data collection: Retinal pigment epithelial cell maturation
├── /Analytical data
│ └── /README1.md.html
│ └── /Codebook.md.html
│ └── /Transcript_expression_analysis.csv
│ └── /Multiplex_immunoassay.csv
│ └── /Multiplex_immunoassay_validation.csv
│ └── /Enzyme_linked_immunosorbent_assay.csv
│ └── /Image_analysis.csv
│ └── /Transepithelial_resistance.csv
├── /Image data
│ └── /README2.md.html
│ └── /Codebook.md.html
│ ├── /Immature_vs_mature.zip
│ ├── /Treatment.zip
│ ├── /Image_analysis.zip
│ └── /Generation_summary_images.ijm
│ └── /Image_analysis_ARPE-19_NA.ijm
│ └── /Image_analysis_CA.ijm
│ └── /Image_analysis_GT335-A.ijm
│ └── /Image_analysis_iPSC-RPE_NA.ijm
├── /Video data
│ └── /README3.md.html
│ └── /Codebook.md.html
│ └── /[Variable(s)]_video.mp4
│ └── /Video_generation.ijm
├── /Western blot data
│ └── /README4.md.html
│ └── /Codebook.md.html
│ ├── /Immature_vs_mature.zip
│ ├── /Treatment.zip
│ ├── /Specificity_controls.zip

Description of mp4-files

Each mp4-file name follows the conventions:

[Variable(s)]_video.mp4