| agC3 |
anti-goat Alexa Cy3 |
| amAF488 |
anti-mouse Alexa Fluor 488 |
| arAF488 |
anti-rabbit Alexa Fluor 488 |
| arAF546 |
anti-rabbit Alexa Fluor 546 |
| ARL13B |
ADP-ribosylation factor-like protein 13B |
| ARL13BandGT335 |
indicates the co-analysis of ARL13B and GT335 |
| BEST1 |
bestrophin 1 |
| C1q |
complement 1q |
| C2 |
complement 2 |
| C3 |
complement 3 |
| C3a |
complement 3a |
| C3antibody1 |
indicates the analysis of C3 with antibody 1 |
| C3antibody3 |
indicates the analysis of C3 with antibody 3 |
| C3aantibody2 |
indicates the analysis of C3/C3a with antibody 2 |
| C3b |
complement 3b |
| C3beta |
complement 3 beta chain |
| C3c |
complement 3c |
| C3d |
complement 3d |
| C3depletedserum |
C3-depleted serum |
| C3(H2O) |
hydrolysed C3 |
| C4 |
complement 4 |
| C4B |
complement 4B |
| C5 |
complement 5 |
| C5a |
complement 5a |
| C9 |
complement 9 |
| CA |
cell body area in pixel per square inch |
| CC |
cell capacitance in micro farad per square centimetre |
| CD |
cell density in cells per square centimetre |
| CDH1 |
cadherin 1 |
| CellType |
indicates if the sample was derived from ARPE-19 or iPSC-RPE cells |
| CFB, FB |
complement factor B |
| CFD, FD |
complement factor D |
| CFH, FH |
complement factor H |
| CFI, FI |
complement factor I |
| Condition |
indicates if the sample belongs to the group of immature, mature, treated, or control cells |
| F-actin |
filamentous actin |
| FHfamily |
indicates the analysis of several members of the FH family |
| FHL-1 |
factor H-like protein 1 |
| FHR-1 |
factor H-related protein 1 |
| FHR-2 |
factor H-related protein 2 |
| FHR-3 |
factor H-related protein 3 |
| FHR-4 |
factor H-related protein 4 |
| FHR-5 |
factor H-related protein 5 |
| GAPDH |
glyceraldehyde-3-phosphate dehydrogenase |
| GT335 |
polyglutamylated tubulin |
| GT335-A |
GT335 ciliary area in pixel per square inch |
| GT335andTJP1 |
indicates the co-analysis of GT335 and TJP1 |
| iC3b |
inactivated complement 3b |
| LAMP2 |
lysosome-associated membrane protein 2 |
| LOG2FC |
log2 fold change |
| MBL |
mannan-binding lectin |
| MERTK |
MER proto-oncogene, tyrosine kinase |
| Method |
“Western blot” indicates that no immune precipitation with the samples was conducted; “Immune precipitation” indicates that immune precipitation was first conducted |
| MFI |
median fluorescence intensity |
| N |
amount of neighbours per cell |
| NA |
nuclear area in pixel per square inch |
| Normalhumanserum |
normal human serum |
| OD |
optical density at 450 nm |
| PMEL |
premelanosome protein |
| RLBP1 |
retinaldehyde binding protein 1 |
| RPE65 |
retinoid isomerohydrolase |
| Sample |
indicates which purified protein or serum sample was used for validation of the multiplex immunoassay |
| SampleType |
indicates if the Western blot sample was cell lysate from immature and mature ARPE-19 and iPSC-RPE cells, cell lysate from treatment cells, culture media, or purified proteins |
| Side |
indicates if the sample was harvested from the apical or the basal side of the cell |
| SNAI1 |
snail family transcriptional repressor 1 |
| TGFBR1, TBRI |
transforming growth factor-beta receptor 1 |
| TGFB1, TGF-Beta1 |
transforming growth factor-beta 1 |
| TGFB1andTBRI |
indicates the co-analysis of TGFB1 and TBRI |
| TER |
transepithelial resistance in ohm square centimetre |
| TJP1 |
tight junction protein 1 |
| VIM |
vimentin |