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Pauly, Diana

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Pauly

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Diana

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Now showing 1 - 3 of 3
  • Research DataOpen Access
    Video data
    (Philipps-Universität Marburg) Schikora, Juliane; Dort, Aaron; Nickel, Antonia; Bergert, Jasmin; Schayan-Araghi, Stella Y.; Banerjee, Pratiti; Wolf, Hannah N.; May-Simera, Helen; Pauly, Diana
    This subset of the repository contains the video data of the study. This includes mp4-files that show stained proteins in an apical to basal direction. It further contains the Fiji macro used for generating mp4-files from tif-files that can be found in the image data subset of this collection.
  • Research DataOpen Access
    Western blot data
    (Philipps-Universität Marburg) Dort, Aaron; Pauly, Diana; Nickel, Antonia; Bergert, Jasmin; Schayan-Araghi, Stella Y.; Banerjee, Pratiti; Wolf, Hannah N.; May-Simera, Helen; Schikora, Juliane
    This subset of the repository contains the Western blot data of the study. The original data comes from mscn-files that were opened using Image Lab which is not in an open free format. Therefore the membrane images were exported as tif-files and the associated metadata was exported as a xml file. The subset contains several replicates of the different experiments and specificity controls conducted with purified proteins, and cell culture media. Furthermore, it contains the Western blot conducted after immune precipitation of cell lysates.
  • Research DataOpen Access
    Image data
    (Philipps-Universität Marburg) Schikora, Juliane; Dort, Aaron; Nickel, Antonia; Bergert, Jasmin; Schayan-Araghi, Stella Y.; Banerjee, Pratiti; Wolf, Hannah N.; May-Simera, Helen; Pauly, Diana
    This subset of the repository contains the image data of the study in zip folders. This includes several replicates of the recorded 16-bit stacks as tif-files of the immunostainings that can be opened using Fiji. It further contains summary images as jpg-files used for image analysis. And it includes Fiji macros used for generation of merged and single channel stacks (Generation_summary_images.ijm), measurement of nuclei area in ARPE-19 cells (Image_analysis_ARPE-19_NA.ijm), nuclei area in iPSC-RPE cells (Image_analysis_iPSC-RPE_NA.ijm), measurement of cell area (Image_analysis_CA.ijm), and measurement of GT335 ciliary area (Image_analysis_GT335-A.ijm).