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May-Simera, Helen

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May-Simera

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    Research DataOpen Access
    Video data
    (Philipps-Universität Marburg) Schikora, Juliane; Dort, Aaron; Nickel, Antonia; Bergert, Jasmin; Schayan-Araghi, Stella Y.; Banerjee, Pratiti; Wolf, Hannah N.; May-Simera, Helen; Pauly, Diana
    This subset of the repository contains the video data of the study. This includes mp4-files that show stained proteins in an apical to basal direction. It further contains the Fiji macro used for generating mp4-files from tif-files that can be found in the image data subset of this collection.
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    Research DataOpen Access
    Image data
    (Philipps-Universität Marburg) Schikora, Juliane; Dort, Aaron; Nickel, Antonia; Bergert, Jasmin; Schayan-Araghi, Stella Y.; Banerjee, Pratiti; Wolf, Hannah N.; May-Simera, Helen; Pauly, Diana
    This subset of the repository contains the image data of the study in zip folders. This includes several replicates of the recorded 16-bit stacks as tif-files of the immunostainings that can be opened using Fiji. It further contains summary images as jpg-files used for image analysis. And it includes Fiji macros used for generation of merged and single channel stacks (Generation_summary_images.ijm), measurement of nuclei area in ARPE-19 cells (Image_analysis_ARPE-19_NA.ijm), nuclei area in iPSC-RPE cells (Image_analysis_iPSC-RPE_NA.ijm), measurement of cell area (Image_analysis_CA.ijm), and measurement of GT335 ciliary area (Image_analysis_GT335-A.ijm).