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Jablonski-Momeni, Anahita

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Jablonski-Momeni

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    Use of BlueCheck on smooth surfaces in-vitro
    Jablonski-Momeni, Anahita
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    In vitro-in situ comparison study - Data set
    (Philipps-Universität Marburg) Jablonski-Momeni, Anahita
    This data set presents the data which were analysed for the following publication: A comparison between in vitro and randomized in situ models for remineralization of artificial enamel lesions Jablonski-Momeni A, Lentz J, Jablonski B, Kiesow A, Morawietz M. Sci Rep. 2024 Oct 25;14(1):25295. doi: 10.1038/s41598-024-76387-w. Abstract: The randomized study aimed to evaluate the comparability of in situ (iS) and in vitro (iV) study protocols regarding remineralization of artificial enamel lesions. Two toothpastes (group A: 1450 ppm sodium fluoride, group B: placebo 0 ppm F-), were investigated. IV, a pH-cycling model with toothpaste slurry treatment was applied for 10d. IS, remineralization was performed in 9 participants wearing splints with embedded enamel samples for 10 and 21d, randomly allocated to groups A and B. Samples were scanned by X-ray micro-computed tomography (μCT) and grayscale value line profiles corresponding to mineral density (rel.ΔZ) were analyzed. T-Test for dependent and independent data and analysis of variance (ANOVA) were used for further analyses (α = 0.05). Rel.ΔZ of fluoride treated samples (A) were iV = 40.2%, iS 10d = 11.5% and iS 21d = 46.1% (p > 0.05). Rel.ΔZ of placebo treated samples (B) were: iV = − 6.2%, iS 10d = 25.2% and iS 21d = 11.0% (p > 0.05). Remineralization potential of both toothpastes was significantly different regarding iV (p < 0.001) and iS after 21d (p = 0.034), while in case of iS 10d no significant difference was detected (p = 0.4). Despite different study protocols the μCT results after remineralization were comparable between iV and iS. The results suggest that selected studies can be carried out in faster, simplified iV studies using pH-cycling instead of iS studies.